Journal: bioRxiv
Article Title: Spatio-molecular gene expression reflects dorsal anterior cingulate cortex structure and function in the human brain
doi: 10.1101/2025.07.14.664821
Figure Lengend Snippet: ( A ) dACC (blue) and dlPFC (pink) regions outlined in midsagittal (dACC) and lateral (dlPFC) brain views, as well as in coronal hemislabs. dACC tissue (blue) used here was sourced from the same ten neurotypical control donors previously profiled for dlPFC (pink) to facilitate within-donor comparisons of dACC agranular cortex versus dlPFC granular cortex (bottom). ( B ) Fresh-frozen coronal brain slab from donor Br3942 taken at the level of the anterior striatum overlaid with the outline of major landmarks from the Atlas of the Human Brain . Three Brodmann areas (BA) 33, 24, and 32 corresponding to the dACC are highlighted in yellow (top left). H&E staining of tissue cryosections confirmed inclusion of dACC on the tissue block (top right). Following anatomical validation, cryosections from 10 neurotypical control donors were collected for Visium and Chromium assays (10x Genomics) from the same block for each donor (bottom).
Article Snippet: To generate SRT data, tissue blocks were vertically scored in ∼6.5 mm strips to match the width of capture areas on the Visium platform (10x Genomics).
Techniques: Control, Staining, Blocking Assay, Biomarker Discovery